Lemna minor as a model organism to evaluate seaweed biostimulants for hydroponic crops

Rodolfo Osin de  Moraes; Bruna Roque Loureiro; Marco Shizuo Owatari; Carolina Antonieta Lopes; Patrícia Soares Santiago; Ana Letícia Madeira Sanches; Levi Pompermayer Machado

doi:10.4136/ambi-agua.3136

Rodolfo Osin de Moraes Instituto de Estudos Avançados do Mar "Prof. Dr. Peter Christian Hackspacher". Departamento de Agronomia e Recursos Naturais. Faculdade de Ciências Agrárias do Vale da Ribeira. Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Câmpus de Registro, Avenida Nelson Brihi Badur, n° 430, CEP: 11900-000, Registro, SP, Brazil.
Bruna Roque Loureiro Natureza Ativa Serviços Ambientais, Avenida Wild José de Souza, n° 456, CEP: 11900-000, Registro, SP, Brazil.
Marco Shizuo Owatari Departamento de Aquicultura. Universidade Federal de Santa Catarina (UFSC), Rodovia Admar Gonzaga, n° 1486, CEP: 88034-000, Florianópolis, SC, Brazil.
Carolina Antonieta Lopes Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC), Rodovia José Carlos Daux, n° 600 (SC-401), km 01, CEP: 88030-902, Florianópolis, SC, Brazil.
Patrícia Soares Santiago Instituto de Estudos Avançados do Mar "Prof. Dr. Peter Christian Hackspacher". Departamento de Agronomia e Recursos Naturais. Faculdade de Ciências Agrárias do Vale da Ribeira. Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Câmpus de Registro, Avenida Nelson Brihi Badur, n° 430, CEP: 11900-000, Registro, SP, Brazil.
Ana Letícia Madeira Sanches Instituto de Estudos Avançados do Mar "Prof. Dr. Peter Christian Hackspacher". Departamento de Recursos Pesqueiros e Aquicultura. Faculdade de Ciências Agrárias do Vale da Ribeira. Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Câmpus de Registro, Avenida Nelson Brihi Badur, n° 430, CEP: 11900-000, Registro, SP, Brazil.
Levi Pompermayer Machado Instituto de Estudos Avançados do Mar "Prof. Dr. Peter Christian Hackspacher". Departamento de Recursos Pesqueiros e Aquicultura. Faculdade de Ciências Agrárias do Vale da Ribeira. Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Câmpus de Registro, Avenida Nelson Brihi Badur, n° 430, CEP: 11900-000, Registro, SP, Brazil.

DOI: https://doi.org/10.4136/ambi-agua.3136

Abstract

Seaweed-based biostimulants (SBB) have emerged as sustainable alternatives for traditional soil-based agriculture and have garnered worldwide attention in recent years. Despite their growing popularity, there is limited information available on the application and efficacy of SBB in hydroponic crops. This study assesses the biostimulant effects of a commercial SBB using Lemna minor as a model organism, developing a standardized bioassay for evaluating SBB in hydroponic cultures. The experiment was carried out under controlled conditions, following a randomized design with 10 treatments. These treatments included SBB (₀T1, _0.5T1, ₂T1) alone at concentrations of 0, 0.5, and 2.0 mg/L, SBB (₀T2, _0.5T2, ₂T2) + Hoagland and Arnon nutrient solution, and SBB (₀T3₀, _0.5T3, ₂T3) + 2.5 g/L NaCl. A control group (₀TC) with distilled water was also included. The results showed that the isolated SBB did not significantly differ (p > 0.05) from the control treatment. Treatments in group T2 had significantly higher coverage area compared to treatments in group T3. The highest absolute percentage of coverage area was observed in 0.5T2 (14.0 ± 1.2%). Regarding the specific growth rate SGR, the higher growth rates were observed in 0.5T2 (0.37 ± 0.02% day⁻1). Group T2 treatments exhibited significantly higher dry biomass (p < 0.05) than group T3 treatments. Treatment with NaCl hindered plant growth. This methodology could enhance the development of a robust protocol for evaluating seaweed biostimulants for hydroponic crops.

Keywords: biostimulants, duckweed, seaweed extract.

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