Potential mutagenic effects of the glyphosate herbicide on Gymnotus inaequilabiatus (Valenciennes, 1839)

  • Larissa Nayara Lima Silva Centro de Pesquisa de Limnologia, Biodiversidade, Etnobiologia do Pantanal. Programa de Pós-graduação em Ciências Ambientais. Universidade do Estado de Mato Grosso (UEM), Cidade Universitária (Bloco II), Avenida Santos Dumont, s/n, CEP: 78200-000, Cáceres, MT, Brazil.
  • Claumir Cesar Muniz Centro de Pesquisa de Limnologia, Biodiversidade, Etnobiologia do Pantanal. Programa de Pós-graduação em Ciências Ambientais. Universidade do Estado de Mato Grosso (UEM), Cidade Universitária (Bloco II), Avenida Santos Dumont, s/n, CEP: 78200-000, Cáceres, MT, Brazil.
  • Ernandes Oliveira Sobreira Junior Centro de Pesquisa de Limnologia, Biodiversidade, Etnobiologia do Pantanal. Programa de Pós-graduação em Ciências Ambientais. Universidade do Estado de Mato Grosso (UEM), Cidade Universitária (Bloco II), Avenida Santos Dumont, s/n, CEP: 78200-000, Cáceres, MT, Brazil.
  • Áurea Regina Alves Ignácio Centro de Pesquisa de Limnologia, Biodiversidade, Etnobiologia do Pantanal. Programa de Pós-graduação em Ciências Ambientais. Universidade do Estado de Mato Grosso (UEM), Cidade Universitária (Bloco II), Avenida Santos Dumont, s/n, CEP: 78200-000, Cáceres, MT, Brazil.
DOI: https://doi.org/10.4136/ambi-agua.2730
Keywords: cell damage, fish, Roundup®.

Abstract

This study analyzed the occurrence of nuclear damage in the red blood cells of Gymnotus inaequilabiatus (Valenciennes, 1839), exposed in vivo to the herbicide glyphosate. The fish were distributed in four groups, namely: control (without addition of herbicide) and contaminated groups with application of 65 µg/L (concentration allowed by CONAMA Resolution), 1 mg/L (maximum limit recommended by the World Health Organization - WHO and Food and Agriculture Organization - FAO) and 5 mg/L (overdose, five times higher than that by WHO and FAO). For the analysis of cell damage, the method of Nuclear Erythrocytic Abnormalities (NEA) was used, containing 1000 cells for duplicate elimination. An increase in cell damage was observed for 144 hours (6-days) of exposure in all controls. Even under a small concentration, there was a clear effect on segmented, renal, lobed formation and mainly on the formation of micronuclei. The high damage was caused in the first 48 hours and reduced after 144 hours, where the fish can have the herbicidal effect blocked. Studies that analyze the mechanisms of action of glyphosate-based herbicides are essential to determine the risks caused by biota, since there is a great divergence on the maximum tolerable limits in water, which affect quality and integrity of these ecosystems.


Published
22/09/2021
Issue
Vol. 16 No. 5 (2021)
Section
Papers

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