Validation of the method for determining the 3 – indolacetic acid in water from rivers by high performance liquid chromatography

Abstract

The 3 – indolyacetic acid is an important natural phytohormone in plants in low concentrations. The 3 – indolyacetic acid is involved in the regulation of many processes of growth and plants development. This study developed and validated an analytical method to quantify 3- indolyacetic acid using the Hight-Performance Liquid Chromatography with DAD detector. The following parameters were chosen: detection and quantification limits, linearity, calibration curve, precision, sensibility and assays of recovery. The calibration curve was obtained with five points based on standard solution. The method presented detection and quantification limits of 0.05 and 0.5 mg L-1, respectively. It showed linearity in the interval of 0.5 to 10 μg L-1 and correlation coefficients higher than 0.99. These results allow the conclusion that the method can be considered efficient and that it could be used to monitor 3 – indolyacetic acid in natural water.