Biodegradation of dairy wastes using crude enzymatic extract of Yarrowia lipolytica ATCC 9773

  • Arnulfo Tarón Dunoyer Faculty of Engineering. University of Cartagena, Campus Piedra de Bolivar. Consulate Avenue, 30th Street, n° 48-152, Cartagena, Colombia.
  • Rafael Emilio González Cuello Faculty of Engineering. University of Cartagena, Campus Piedra de Bolivar. Consulate Avenue, 30th Street, n° 48-152, Cartagena, Colombia.
  • Rosangela Perez Salinas Faculty of Health Sciences. University of Santander, Center neighborhood, 6th Race, n° 14-27, Valledupar, Colombia.
DOI: https://doi.org/10.4136/ambi-agua.2448
Keywords: biological treatment, fatty effluents, removal, Y lipolytica

Abstract

 

Effluents generated by the food industry have become a serious environmental concern. Bioremediation is a biological process developed as an alternative for the treatment of contaminated areas. In current research, the biodegradation of fat, Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD) and total solids were evaluated in dairy waste employing enzymatic extract of Yarrowia lipolytica ATCC 9773 as biological agents. All the variables were determined following the specifications of the Standard Methods of the American Water Works Association. Enzymatic extract of Y. lipolytica at different concentrations (8, 12 and 16.0%) was used in a fermentative medium at two pHs (5.0 and 6.5) for 32 h. The highest percentages (%) of fat (82.88), BOD (43.32), COD (44.3) and total solids (13.58) removal were obtained using an inoculum concentration of 16% at pH 5.0 for 32 h of fermentation. These results may have industrial relevance for the reduction of contamination of industrial effluents with high levels of fat and other contaminants.


Author Biographies

Arnulfo Tarón Dunoyer, Faculty of Engineering. University of Cartagena, Campus Piedra de Bolivar. Consulate Avenue, 30th Street, n° 48-152, Cartagena, Colombia.
     
Rafael Emilio González Cuello, Faculty of Engineering. University of Cartagena, Campus Piedra de Bolivar. Consulate Avenue, 30th Street, n° 48-152, Cartagena, Colombia.
    
Rosangela Perez Salinas, Faculty of Health Sciences. University of Santander, Center neighborhood, 6th Race, n° 14-27, Valledupar, Colombia.
   
Published
14/02/2020
Issue
Vol. 15 No. 1 (2020)
Section
Papers

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